PIGC Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

PIGC Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PIGC.
  
  

  Source: E. coli

  Amino Acid Sequence: YLIRLQLFKENIHGPWDEAEIKEDLSRFLS

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: PIGC
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-86283.It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for PIGC Recombinant Protein Antigen

• EC 2.4.1.198
• GPI2class C protein
• MGC2049
• phosphatidylinositol glycan anchor biosynthesis, class C
• phosphatidylinositol glycan, class C

Background

PIGC, also known as Phosphatidylinositol N-acetylglucosaminyltransferase subunit C, is 297 amino acids in length and approximately 34 kDa. PIGC is an important player in GPI anchor biosynthesis because it catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol. Current research on PIGC is being performed relating to several diseases and disorders including malaria and lassa fever. PIGC has also been shown to have interactions with PIGA, DPM2, KLF10, PIGQ and ZHX1 in pathways such as GPI anchor biosynthesis and metabolic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PIGC