PICK1 Antibody

Images

 
Western Blot: PICK1 Antibody [NB120-3420] - Analysis of PICK1 from AtT20 cell lysate.
Immunocytochemistry/ Immunofluorescence: PICK1 Antibody [NB120-3420] - Immunolocalization of PICK1 in CV1 cells transfected with both PICK1 and mGluR7a.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PICK1 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1.
Localization
Cytoplasmic; concentrated in the perinuclear region. Also present at excitatory synapses.
Specificity
Detects protein interacting with Protein Kinase C (PICK 1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PICK1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry 1.7 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
WB: Detects an approx. ~52 kDa protein representing PICK 1 from AtT20 cell extract. ICC: Staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
Publications
Read Publication using NB120-3420.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PICK1 Antibody

  • alpha binding protein
  • dJ1039K5
  • MGC15204
  • PICK
  • PRKCA-binding protein
  • PRKCABPprotein interacting with PRKCA
  • Protein interacting with C kinase 1
  • protein interacting with PRKCA 1
  • Protein kinase C-alpha-binding protein

Background

PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PICK1 Antibody (NB120-3420)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PICK1 Antibody (NB120-3420). (Showing 1 - 1 of 1 FAQ).

  1. Here is a difficult request. We are looking for an antibody with the following specifications: Anti-PICK1 Reactive in mouse without sodium azide, so it can be injected into the mouse without compromising its life With PBS (for example) It does not necessarily have to be guaranteed. Have you got anything like it?
    • Unfortunately all of our anti-PICK1 mouse-reactive antibodies are supplied in sodium azide. We do have anti human PICK1 without preservatives, but that would not be suitable if you are working with mouse samples. NB100-41403 contains only 0.02% sodium azide which is the lowest percentage. We also offer an Antibody Concentration and Clean Up Antibody Purification Kit which can be used to reduce the concentration of unwanted additives often found in antibody formulations, such as sodium azide, glycine or tris. This kit is offered in two sizes: cat# 861-0005 includes 1 column and cat# 861-0010 includes 3 columns.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PICK1
Entrez