Phospholamban Antibody

Western Blot: Phospholamban Antibody [NBP1-49852] - Analysis of Phospholamban in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-49852 at 0.03ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-49852] - 3.75ug/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Phospholamban Antibody Summary

• Immunogen: Peptide with sequence KVQYLTRSAIRR-C corresponding to N-Terminus according to NP_002658.1.
• Epitope: KVQYLTRSAIRR - N Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.01-0.03ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:1000
• Application Notes: Western blot: Approx 13kDa band observed in Human and Rat Heart lysates (calculated MW of 6.11kDa according to NP_002658.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Heart.

Reactivity Notes

Expected from sequence similarity: Porcine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Phospholamban Antibody

• CMD1PPLBcardiac phospholamban
• phospholamban

Background

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phospholamban Antibody (NBP1-49852)(1)

Publication using NBP1-49852

• Moller DV, Pham TT, Gustafsson F et al. The role of Lamin A/C mutations in Danish patients with idiopathic dilated cardiomyopathy. Eur J Heart Fail 2009-11-01 [PMID: 19875404]

Bioinformatics

• Gene Symbol: PLN
• Entrez:
  • 5350(Human)
  • 64672(Rat)
• Uniprot:
  • NP_002658.1(Human)