Phosphodiesterase 4D Antibody

Product Discontinued

Phosphodiesterase 4D Antibody Summary

• Immunogen: C-terminal cyclic peptide unique to all PDE4D subtypes variants.
• Specificity: This antibody labels only PDE4D1 variant in rat cortical membranes, the antibody does not cross reacts with PDE4D3, D2, D4 and D5. Does not cross react with PDE4A, PDE4B or PDE4C.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE4D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:10-1;2000
  • Immunohistochemistry 1:100-1:250
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES, BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Phosphodiesterase 4D Antibody

• cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E3)
• cAMP-specific 3'-5'-cyclic phosphodiesterase 4D
• cAMP-specific phosphodiesterase PDE4D6
• DKFZp686M11213
• DPDE3
• Drosophila)
• dunce-like phosphodiesterase E3
• EC 3.1.4
• FLJ97311
• HSPDE4D
• PDE43
• PDE4D
• Phosphodiesterase 4D
• phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog
• phosphodiesterase 4D, cAMP-specific

Background

Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE4D
• Entrez:
  • 5144(Human)
  • 238871(Mouse)
  • 24627(Rat)