PHOSPHO1 Antibody (4B2) - Azide and BSA Free

Western Blot: PHOSPHO1 Antibody (4B2) [H00162466-M04] - Analysis of PHOSPHO1 expression in PC-12 (Cat # L012V1).

Western Blot: PHOSPHO1 Antibody (4B2) [H00162466-M04] - Analysis of PHOSPHO1 expression in Jurkat (Cat # L017V1).

ELISA: PHOSPHO1 Antibody (4B2) [H00162466-M04] - Detection limit for recombinant GST tagged PHOSPHO1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, DB, ELISA
• Clone: 4B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PHOSPHO1 Antibody (4B2) - Azide and BSA Free Summary

• Immunogen: PHOSPHO1 (NP_848595, 168 a.a. ~ 266 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKS
• Specificity: PHOSPHO1 - phosphatase, orphan 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHOSPHO1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PHOSPHO1 Antibody (4B2) - Azide and BSA Free

• EC 3.1.3.75
• phosphatase, orphan 1
• phosphoethanolamine/phosphocholine phosphatase

Background

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). May beinvolved in the generation of inorganic phosphate for bone mineralization

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHOSPHO1 Antibody (H00162466-M04)(1)

Publication using H00162466-M04

• Luthringer BJ, Katha UM, Willumeit R. Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis. J Mater Sci Mater Med. 2014-07-01 [PMID: 24980874]

Bioinformatics

• Gene Symbol: PHOSPHO1
• Entrez:
  • 162466(Human)
• Uniprot:
  • NP_848595(Human)