PHLPP2 Antibody [DyLight 550]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 550

PHLPP2 Antibody [DyLight 550] Summary

• Immunogen: PHLPP2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PHLPP2. The immunogen is located within the last 50 amino acids of PHLPP2. Amino Acid Squence: DSRLEPEPHEEDRTEPPEEF
• Specificity: At least three isoforms are known to exist; this antibody will detect the two largest isoforms. PHLPP2 antibody is predicted to not cross react with PHLPP1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHLPP2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for PHLPP2 Antibody [DyLight 550]

• EC 3.1.3.16
• KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase-like
• PH domain leucine-rich repeat-containing protein phosphatase-like
• PHLPPLPHLPP-like

Background

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHLPP2