PHACTR3 Antibody - BSA Free

Western Blot: PHACTR3 Antibody [NBP3-12697] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: PHACTR3 Antibody [NBP3-12697] - Staining of human PHACTR3 in human brain with rabbit polyclonal antibody at 1:100 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PHACTR3 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PHACTR3 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHACTR3
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PHACTR3 Antibody - BSA Free

• C20orf101
• chromosome 20 open reading frame 101
• H17739
• MGC117178
• phosphatase and actin regulator 3
• scaffold-associated PP1 inhibiting protein
• Scaffold-associated PP1-inhibiting protein
• SCAPIN1
• SCAPININ

Background

PHACTR3 is encoded by this gene is associated with the nuclear scaffold in proliferating cells. It was found to bind to the catalytic subunit of protein phosphatase-1 (PP1) and inhibit PP1 activity, suggesting that this protein may function as a regulatory subunit of PP1. Alternative splicing at this locus results in several transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHACTR3