Peroxiredoxin 6 Antibody

Product Discontinued

Peroxiredoxin 6 Antibody Summary

• Immunogen: Rat recombinant Peroxiredoxin-6. The sequence is homologous in human and mouse peroxiredoxin-6.
• Localization: Accession Number: PRDX6_RAT ; PRDX6_HUMAN ; PRDX6_MOUSE
• Specificity: This has been shown to be specific for Peroxiredoxin-6 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRDX6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: IHC-P, WB, ELISA. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary.
• Control:

  Peroxiredoxin 6 Overexpression Lysate

Reactivity Notes

Rat, human and mouse, other species have not yet been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with deionized water.

Alternate Names for Peroxiredoxin 6 Antibody

• 1-Cys
• 24 kDa protein
• Acidic calcium-independent phospholipase A2
• aiPLA2
• aiPLA21-Cys PRX
• Antioxidant protein 2
• AOP2
• AOP2Non-selenium glutathione peroxidase
• EC 1.11.1.15
• EC 1.11.1.7
• EC 3.1.1.-
• KIAA0106Red blood cells page spot 12
• Liver 2D page spot 40
• MGC46173
• NSGPx
• NSGPx1-Cys peroxiredoxin
• p29
• Peroxiredoxin 6
• peroxiredoxin-6
• PRDX6
• PRX

Background

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 6 Antibody (R-169-100)(1)

Publication using R-169-100

• 1Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M. (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc;5(1):31-8. 2010-01-01 [PMID: 20057379]

Bioinformatics

• Gene Symbol: PRDX6
• Entrez:
  • 9588(Human)
  • 11758(Mouse)
  • 94167(Rat)