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Human Pepsinogen A ELISA Kit (Colorimetric)

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ELISA: Human Pepsinogen A ELISA Kit (Colorimetric) [NBP2-81244] - Standard Curve Reference
ELISA: Human Pepsinogen A ELISA Kit (Colorimetric) [NBP2-81244] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids.
Standard Curve Range
12.5 - 800 pg/mL
Sensitivity
7.5 pg/mL

Order Details

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Human Pepsinogen A ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Pepsinogen A in samples. No significant cross-reactivity or interference between Human Pepsinogen A and analogues was observed.
Standard Curve Range
12.5 - 800 pg/mL
Sensitivity
7.5 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.62%
Intra-Assay
CV% < 5.51%
Spike Recovery
90-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PGA4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human Pepsinogen A ELISA Kit (Colorimetric)

  • EC 3.4.23
  • EC 3.4.23.1
  • FLJ58952
  • FLJ77962
  • pepsin A
  • pepsinogen 4, group I (pepsinogen A)
  • Pepsinogen A
  • pepsinogen A4
  • PGA3
  • PGA5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog&amp;nbsp;# AF7094) and NorthernLights&amp;#8482; 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog&amp;nbsp;# NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(5 Reviews)
(28 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Ovalbumin Antibody
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Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin. Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.
Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of purified egg white proteins (lysozyme, ovalbumin, ovotransferrin). Image from verified customer review.
(7 Publications)
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Applications: ELISA, IHC, IHC-P, IP, Single-Cell Western, WB
Secretin Antibody
NBP2-48988
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining in human duodenum and liver tissues using anti-SCT antibody. Corresponding SCT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining of human liver shows low expression as expected.
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Applications: IHC, IHC-P
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H00002520-P01
SDS-Page: Recombinant Human Gastrin peptide Protein [H00002520-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
(2 Publications)
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Applications: ELISA, AP, PA, PAGE, WB
Insulin Antibody
(182410) [Unconjugate ...
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Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2&amp;nbsp;µg/mL of Sheep Anti-Human GIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8060) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog&amp;nbsp;# HAF016). A specific band was detected for GIF at approx&amp;shy;imately&amp;nbsp;48-52&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Cholecystokinin
Antibody
NBP2-62664
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Analysis in human duodenum and skeletal muscle tissues using Anti-CCK antibody. Corresponding CCK RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human skeletal muscle shows low expression as expected.
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TIMP-1 [HRP]
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Pepsinogen
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Renin was detected in immersion fixed paraffin-embedded sections of human kidney array using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Sheep HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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LAP3 Antibody
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Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847] - Staining in human lymph node and skeletal muscle tissues using anti-LAP3 antibody. Corresponding LAP3 RNA-seq data are presented for the same tissues.
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Immunohistochemistry-Paraffin: Myoglobin Antibody (SC06-38) [NBP2-67599] - Analysis of paraffin-embedded mouse heart tissue using anti-Myoglobin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Myoglobin Antibody (SC06-38) [NBP2-67599] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myoglobin antibody. Counter stained with hematoxylin.
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Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Catalase at approximately 62&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50&amp;nbsp;dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for Catalase at approximately 64&amp;nbsp;kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog&amp;nbsp;# AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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POMC Antibody
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Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
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Cathepsin&amp;nbsp;D was detected in perfusion fixed frozen sections of mouse intestine using 15&amp;nbsp;µg/mL Goat Anti-Mouse Cathepsin&amp;nbsp;D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) overnight at 4&amp;nbsp;°C. Tissue was stained with the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog # CTS008) and counter&amp;shy;stained with hematoxylin (blue). Specific labeling was localized to epithelial cells in intestinal villi. View&amp;nbsp;our&amp;nbsp;protocol&amp;nbsp;for Chromogenic IHC Staining of Frozen Tissue Sections.
Cathepsin&amp;nbsp;D was detected in immersion fixed RAW&amp;nbsp;264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse Cathepsin&amp;nbsp;D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) at 5&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysate of mouse intestine tissue. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Goat Anti-Mouse Cathepsin&amp;nbsp;D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). Specific bands were detected for Cathepsin&amp;nbsp;D at approximately 28 and 45&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Lysozyme Antibody
- BSA Free
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Simple Western: Lysozyme Antibody [NBP2-61118] - Lane view shows lysates of Ileum stem cells undifferentiated and day 5 differentiated, loaded at 0.2 mg/mL. A specific band was detected for Lysozyme at approximately 20 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Lysozyme Polyclonal Antibody (NBP2-61118). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Flow Cytometry: Lysozyme Antibody [NBP2-61118] - An intracellular stain was performed on THP-1 Cells with NBP2-61118 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&amp;D Systems, F0111).
Immunocytochemistry/Immunofluorescence: Lysozyme Antibody [NBP2-61118] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Lysozyme NBP2-61118 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
(4 Publications)
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Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Fibronectin
Antibody - BSA Free
NBP1-91258
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.
(11 Reviews)
(38 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB

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Bioinformatics

Gene Symbol PGA4