PDLIM7 Antibody (2D6) - Azide and BSA Free

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - PDLIM7 monoclonal antibody (M01), clone 2D6 Analysis of PDLIM7 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of monoclonal antibody to PDLIM7 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 over-expressed 293 cell line, cotransfected with PDLIM7 Validated Chimera RNAi ( Cat # H00009260-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PDLIM7 monoclonal antibody (M01), clone 2D6 (Cat # H00009260-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 expression in transfected 293T cell line by PDLIM7 monoclonal antibody (M01), clone 2D6.Lane 1: PDLIM7 transfected lysate(49.8 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 transfected lysate using anti-PDLIM7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PDLIM7 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: PDLIM7 Antibody (2D6) [H00009260-M01] - Detection limit for recombinant GST tagged PDLIM7 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IP, KD
• Clone: 2D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PDLIM7 Antibody (2D6) - Azide and BSA Free Summary

• Immunogen: PDLIM7 (AAH01093.1, 1 a.a. ~ 457 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGVPYCERDYEKMFGTKCHGCDFKIDAGDRFLEALGFSWHDTCFVCAICQINLEGKTFYSKKDRPLCKSHAFSHV
• Specificity: PDLIM7 - PDZ and LIM domain 7 (enigma)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDLIM7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDLIM7 Antibody (2D6) - Azide and BSA Free

• 1110003B01Rik
• ENIGMAProtein enigma
• LIM domain protein
• LIM mineralization protein
• LMP
• LMP1
• PDZ and LIM domain 7 (enigma)
• PDZ and LIM domain protein 7

Background

The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDLIM7 Antibody (H00009260-M01)(1)

Publication using H00009260-M01

• Herrick S, Evers DM, Lee JY et al. Postsynaptic PDLIM5/Enigma Homolog binds SPAR and causes dendritic spine shrinkage. Mol Cell Neurosci. 2009-11-10 [PMID: 19900557]

Bioinformatics

• Gene Symbol: PDLIM7
• Entrez:
  • 9260(Human)
• OMIM:
  • 605903(Human)
• Uniprot:
  • AAH01093(Human)