PDK-1 Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Pm, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

PDK-1 Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to an internal portion of the human PDK1 protein sequence (between residues 300-400). [Swiss-Prot Q15118]
Localization
Mitochondrion
Predicted Species
Mouse (100%), Rat (100%), Porcine (91%), Bovine (100%), Zebrafish (91%), Chicken (92%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This PDK1 antibody is useful for Western blot, where a band is seen at ~49 kDa representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Predicted to react with rat, mouse, primate and bovine based on 100% sequence homology. Immunogen sequence has 92% homology to chicken, 91% homology to Zebrafish and porcine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PDK-1 Antibody [DyLight 405]

  • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
  • EC 2.7.11
  • EC 2.7.11.2
  • mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
  • PDK1
  • PDK-1
  • PDPK1
  • PKB kinase
  • Pyruvate dehydrogenase kinase isoform 1
  • pyruvate dehydrogenase kinase, isoenzyme 1
  • pyruvate dehydrogenase kinase, isozyme 1

Background

PDK1 (pyruvate dehydrogenase kinase isoform 1) belongs to PDK/BCKDK protein kinase family and is a serine/threonine kinase which regulates glucose and fatty acid metabolism/homeostasis via phosphorylation of pyruvate dehydrogenase (PDH) subunits PDHA1 and PDHA2. PDK1 is mainy expressed in cardiac tissue and it localizes to mitochondrion matrix where it phosphoryles E1 alpha subunit of mitochondrial PDH complex, leading to PDH inhibition thus contributing to the regulation of glucose metabolism. PDK1 inhibits PDH activity, and thus regulates metabolite flux through the TCA/Kreb's cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Because, PDH is a gatekeeper enzyme that converts pyruvate to acetyl-CoA, PDK1 is also regarded as a key regulator of the Warburg effect. PDK1 plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. It is inhibited by AZD7545, dichloroacetate and radicicol and these inhibitors have been tested for anticancer activity. Knockdown of PDK1 by shRNA lowers PDH phosphorylation, reactivates PDC activity and decreases invasiveness in human head and neck squamous cell carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PDK1
Entrez