PDE4B Antibody (SR2301) - BSA Free

Western Blot: PDE4B Antibody (SR2301) [NBP3-22387] - Western blot analysis using NBP3-22387 in A549 cell lysate.

• Reactivity: Hu, Mu
• Applications: WB, Flow
• Clone: SR2301
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PDE4B Antibody (SR2301) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human PDE4B (Uniprot #: Q07343)
• Specificity: Detects endogenous levels of total PDE4B
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDE4B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PDE4B Antibody (SR2301) - BSA Free

• cAMP-specific (phosphodiesterase E4 dunce homolog
• cAMP-specific 3'-5'-cyclic phosphodiesterase 4B
• cAMP-specific phosphodiesterase-4 B isoform
• DKFZp686F2182
• DPDE4
• Drosophila)
• dunce-like phosphodiesterase E4
• EC 3.1.4
• EC 3.1.4.17
• MGC126529
• PDE32
• PDE4B
• PDEIVB
• phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E4)
• phosphodiesterase 4B, cAMP-specific (dunce
• phosphodiesterase 4B, cAMP-specific
• phosphodiesterase E4 dunce homolog

Background

PDE4B is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) make up the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) family consists of four known splice variants, PDE4B1, B2, B3 and a novel 66 kDa PDE4B4 protein. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4B variants are also substrate for PKA-dependent phosphorylation. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants; the PDE4B4 (66kDa protein) is the major PDE4B variant expressed in lungs, testis and heart. In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE4B