PDE3A Antibody

Images

 
Western Blot: PDE3A Antibody [NBP2-19744] - Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5 nM and 25 nM resulted in ~30% protein reduction ...read more
Western Blot: PDE3A Antibody [NBP2-19744] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PDE3A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PDE3A. The exact sequence is proprietary.
Localization
Membrane; Peripheral membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:3000
Application Notes
PDE3A antibody validated for WB from a verified customer review.
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PDE3A Knockout HeLa Cell Lysate
PDE3A Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP2-19744 in the following application:

Reactivity Notes

Pig (88%). Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for PDE3A Antibody

  • CGI-PDE A
  • CGI-PDE
  • cGMP-inhibited 3'-5'-cyclic phosphodiesterase A
  • cyclic GMP inhibited phosphodiesterase A
  • Cyclic GMP-inhibited phosphodiesterase A
  • EC 3.1.4
  • EC 3.1.4.17
  • phosphodiesterase 3A, cGMP-inhibited

Background

Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents (Meacci et al., 1992 [PubMed 1315035]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PDE3A Antibody (NBP2-19744) (0)

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Review for PDE3A Antibody (NBP2-19744) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using NBP2-19744:
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Images Ratings Applications Species Date Details
Western Blot PDE3A NBP2-19744
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4
reviewed by:
YINZHOU ZHU
WB Human and Rat 01/10/2020
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Summary

ApplicationWestern Blot
Sample Tested293T lysates,H9c2 whole cell lysate
SpeciesHuman and Rat
Lot40779

Comments

CommentsAntibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5nM and 25nM resulted in ~30% protein reduction (lanes 2, 3 vs lanes 5, 6).
Ab dilution: 1:1500
Secondary incubation: 1:10000
Blocking: 5% BSA

Product General Protocols

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Video Protocols

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FAQs for PDE3A Antibody (NBP2-19744) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

YINZHOU ZHU
01/10/2020
Application: WB
Species: Human and Rat

Bioinformatics

Gene Symbol PDE3A
Uniprot