Western Blot: PDE3A Antibody [NBP2-19744] - Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5 nM and 25 nM resulted in ~30% protein reduction ...read more
Western Blot: PDE3A Antibody [NBP2-19744] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:1000.
PDE3A antibody validated for WB from a verified customer review.
125 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Pig (88%). Rat reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol, 1% BSA
Immunogen affinity purified
Alternate Names for PDE3A Antibody
cGMP-inhibited 3'-5'-cyclic phosphodiesterase A
cyclic GMP inhibited phosphodiesterase A
Cyclic GMP-inhibited phosphodiesterase A
phosphodiesterase 3A, cGMP-inhibited
Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents (Meacci et al., 1992 [PubMed 1315035]).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for PDE3A Antibody (NBP2-19744) (0)
There are no publications for PDE3A Antibody (NBP2-19744).
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Review for PDE3A Antibody (NBP2-19744) (1)
Average Rating: 4 (Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.
Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5nM and 25nM resulted in ~30% protein reduction (lanes 2, 3 vs lanes 5, 6). Ab dilution: 1:1500 Secondary incubation: 1:10000 Blocking: 5% BSA
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.