PDE3A Antibody

Western Blot: PDE3A Antibody [NBP2-19744] - Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5 nM and 25 nM resulted in ~30% protein reduction (lanes 2, 3 vs lanes 5, 6). Loading of all 293T cell lysates are ~30 ug. H9C2 lysate is ~7 ug due to limited materials. Antibody at 1:500. Secondary antibody at 1:10,000. Blocked with 5% BSA. WB image submitted by a verified customer review.

Western Blot: PDE3A Antibody [NBP2-19744] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDE3A Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PDE3A. The exact sequence is proprietary.
• Localization: Membrane; Peripheral membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:3000
• Application Notes: PDE3A antibody validated for WB from a verified customer review.
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PDE3A Knockout HeLa Cell Lysate
  PDE3A Knockout HeLa Cell Lysate

Reactivity Notes

Pig (88%). Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PDE3A Antibody

• CGI-PDE A
• CGI-PDE
• cGMP-inhibited 3'-5'-cyclic phosphodiesterase A
• cyclic GMP inhibited phosphodiesterase A
• Cyclic GMP-inhibited phosphodiesterase A
• EC 3.1.4
• EC 3.1.4.17
• phosphodiesterase 3A, cGMP-inhibited

Background

Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents (Meacci et al., 1992 [PubMed 1315035]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for PDE3A Antibody (NBP2-19744) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human and Rat.

reviewed by: YINZHOU ZHU WB Human and Rat 01/10/2020

Summary

• Application: Western Blot
• Sample Tested: 293T lysates,H9c2 whole cell lysate
• Species: Human and Rat
• Lot: 40779

Comments

• Comments: Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5nM and 25nM resulted in ~30% protein reduction (lanes 2, 3 vs lanes 5, 6).
  Ab dilution: 1:1500
  Secondary incubation: 1:10000
  Blocking: 5% BSA

Bioinformatics

• Gene Symbol: PDE3A
• Uniprot:
  • Q14432(Human)