PDE3A Antibody

Western Blot: PDE3A Antibody [NBP1-46182] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3A antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PDE3A was also immunoprecipitated by rabbit anti-PDE3A antibody NBP1-46181, which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PDE3A Antibody Summary

• Description: Novus Biologicals Rabbit PDE3A Antibody (NBP1-46182) is a polyclonal antibody validated for use in WB and IP. Anti-PDE3A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 575 and 625 of human Phosphodiesterase 3A using the numbering given in entry NP_000912.3 (GeneID 5139).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE3A Antibody

• CGI-PDE A
• cGI-PDE
• cGMP-inhibited 3'-5'-cyclic phosphodiesterase A
• cyclic GMP inhibited phosphodiesterase A
• Cyclic GMP-inhibited phosphodiesterase A
• EC 3.1.4
• EC 3.1.4.17
• PDE3A
• phosphodiesterase 3A, cGMP-inhibited

Background

Phosphodiesterase 3A is a cGMP inhibited, cAMP/cGMP dual-specific phosphodiesterase. PDE3A has been implicated in male erectile dysfunction, mammalian oocyte maturation, platelet function, vascular smooth muscle function, and salivary gland tumors. Three alternatively spliced transcripts have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDE3A Antibody (NBP1-46182)(1)

Publication using NBP1-46182

• Yu B, Shen K, Li T et al. Glycolytic enzyme PFKFB3 regulates sphingosinae 1-phosphate receptor 1 in proangiogenic glomerular endothelial cells under diabetic condition American journal of physiology. Cell physiology 2023-10-02 [PMID: 37781737] (WB, Mouse)

Bioinformatics

• Gene Symbol: PDE3A