PDE1B Antibody (OTI6E4) [PE/Cy5.5]

Product Discontinued

PDE1B Antibody (OTI6E4) [PE/Cy5.5] Summary

• Immunogen: Full length recombinant protein of human PDE1B (NP_000915) produced in HEK293T cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDE1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

Alternate Names for PDE1B Antibody (OTI6E4) [PE/Cy5.5]

• 63 kDa Cam-PDE
• calcium/calmodulin-dependent 3'-5'-cyclic nucleotide phosphodiesterase 1B
• Cam-PDE 1B
• EC 3.1.4
• EC 3.1.4.17
• PDE1B
• PDE1B1calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase
• PDES1B
• PDES1Bcalmodulin-stimulated phosphodiesterase PDE1B1
• phosphodiesterase 1B, calmodulin-dependent
• phosphodiesterase IB, calmodulin-dependent
• Phosphodiesterase-1B
• presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase familyknown as PDE1B

Background

PDE1B, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The tissue distribution of PDE1B is different from other PDE1 gene family members. In testis PDE1B is expressed uniformly through out the seminiferous epithelium and in the interstistium in contrast to PDE1A and PDE1C that is expressed in elongated to mature spermatids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE1B