Pax3 Antibody [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 669

Pax3 Antibody [Janelia Fluor® 669] Summary

• Immunogen: E. coli-derived recombinant human Pax3
  Met1-Ser215
  Accession # NP_000429
• Specificity: Detects Pax3 in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human (rh) Pax5 and 1% rhPax7, under reducing conditions, is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAX3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Pax3 Antibody [Janelia Fluor® 669]

• CDHS
• HUP2
• HUP2MGC120384
• MGC120381
• MGC120382
• MGC120383
• MGC134778
• paired box 3
• paired box gene 3 (Waardenburg syndrome 1)
• paired box homeotic gene 3
• paired box protein Pax-3
• paired domain gene 3
• paired domain gene HuP2
• Pax3
• Waardenburg syndrome 1
• WS1
• WS3

Background

PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX3