PAR2 Antibody (344222) [Unconjugated]

HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 344222
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PAR2 Antibody (344222) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human PAR2
  Accession # P55085
• Specificity: Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: F2RL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAR2 Antibody (344222) [Unconjugated]

• CFIIRL1
• coagulation factor II (thrombin) receptor-like 1
• Coagulation factor II receptor-like 1
• F2RL1
• GPCR11
• GPR11protease-activated receptor 2
• G-protein coupled receptor 11
• PAR2
• PAR-2
• PAR2G protein-coupled receptor-11
• proteinase-activated receptor 2
• proteinase-activated receptor-2
• Thrombin receptor-like 1

Background

Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAR2 Antibody (MAB3949)(1)

Publication using MAB3949

• RKY Cheng, C Fiez-Vanda, O Schlenker, K Edman, B Aggeler, DG Brown, GA Brown, RM Cooke, CE Dumelin, AS Doré, S Geschwindn, C Grebner, NO Hermansson, A Jazayeri, P Johansson, L Leong, R Prihandoko, M Rappas, H Soutter, A Snijder, L Sundström, B Tehan, P Thornton, D Troast, G Wiggin, A Zhukov, FH Marshall, N Dekker Structural insight into allosteric modulation of protease-activated receptor 2 Nature, 2017;545(7652):112-115. 2017 [PMID: 28445455] (Binding Model, Human)

Bioinformatics

• Gene Symbol: F2RL1
• Uniprot:
  • P55085()