PACSIN1 Antibody

Western Blot: PACSIN1 Antibody [NB100-861] - Staining of hippocampus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PACSIN1 Antibody [NB100-861] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: PACSIN1 Antibody [NB100-861] - Staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PACSIN1 Antibody Summary

• Immunogen: Peptide with sequence SSSYDEASLAPEET-C corresponding to N-Terminus according to NP_065855.1.
• Specificity: Reported variants represent identical protein (NP_065855.1; NP_001186512.1).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PACSIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human brain (hippocampus) lysates (calculated MW of 51.1 kDa band according to NP_065855.1). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Brain (Cortex and Cerebellum).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PACSIN1 Antibody

• KIAA1379SDPI
• protein kinase C and casein kinase substrate in neurons 1
• protein kinase C and casein kinase substrate in neurons protein 1
• syndapin I

Background

PACSINs are a family of cytoplasmic phosphoproteins that play a role in vesicle formation and transport. Highly expressed in brain and to a lesser extent in heart, pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PACSIN1 Antibody (NB100-861)(1)

Publication using NB100-861

• Modregger J, DiProspero NA, Charles V et al. PACSIN 1 interacts with huntingtin and is absent from synaptic varicosities inpresymptomatic Huntington's disease brains. Hum Mol Genet 2002-10-01 [PMID: 12354780]

Bioinformatics

• Gene Symbol: PACSIN1
• Entrez:
  • 29993(Human)
• Uniprot:
  • NP_065855(Human)