PACAP/ADCYAP1 Antibody [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

PACAP/ADCYAP1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant mouse PACAP/ADCYAP1
  Pro26-Leu175
  Accession # O70176
• Specificity: Detects mouse PACAP/ADCYAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 12% cross‑reactivity with recombinant human PACAP is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PACAP/ADCYAP1 Antibody [Alexa Fluor® 700]

• ADCYAP1
• adenylate cyclase activating polypeptide 1 (pituitary)
• PACAP
• PACAPMGC126852
• pituitary adenylate cyclase-activating polypeptide

Background

PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17 kDa (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides. It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes. PACAP has multiple effects. On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and alpha ‑MSH. Mouse PACAP proprecursor is 151 amino acids (aa) in length (aa 25‑175). It undergoes proteolytic processing to generate two C‑terminal amidated peptides termed PACAP-38 (aa 131‑168) and PACAP-27 (aa 131‑157). In general, PACAP-38 represents the dominant form. However, the ratio between the -38 and -27 forms differs in a cell-specific manner. Over aa 25‑175, mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.