PAC2 Antibody (OTI1E8)

Western Blot: PAC2 Antibody (1E8) [NBP2-45438] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAC2.

Immunohistochemistry: PAC2 Antibody (1E8) [NBP2-45438] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: PAC2 Antibody (1E8) [NBP2-45438] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: PAC2 Antibody (1E8) [NBP2-45438] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: PAC2 Antibody (1E8) [NBP2-45438] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: PAC2 Antibody (1E8) [NBP2-45438] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: PAC2 Antibody (1E8) [NBP2-45438] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PAC2 Antibody (OTI1E8) Summary

• Immunogen: Full length human recombinant protein of human PSMG2 (NP_064617) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
• Theoretical MW: 29.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAC2 Antibody (OTI1E8)

• CLAST3HSPC260
• HCCA3PAC-2
• hepatocellular carcinoma susceptibility protein
• Hepatocellular carcinoma-susceptibility protein 3
• HsT1707
• likely ortholog of mouse CD40 ligand-activated specific transcript 3 (Clast3)
• MDS003
• MGC15092
• PAC2HDCMC29P
• proteasome (prosome, macropain) assembly chaperone 2
• proteasome assembling chaperone 2
• proteasome assembly chaperone 2
• TNFSF5IP1CD40 ligand-activated specific transcript 3
• Tumor necrosis factor superfamily member 5-induced protein 1
• tumor necrosis factor superfamily, member 5-induced protein 1
• x 003 protein

Background

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMG2