p70 S6 Kinase beta/S6K2 Knockout 293T Cell Lysate

Product Discontinued

p70 S6 Kinase beta/S6K2 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,179 bp deletion in exon 1
• Gene: RPS6KB2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for p70 S6 Kinase beta/S6K2 Knockout 293T Cell Lysate

• EC 2.7.11
• EC 2.7.11.1
• KLS
• p70 ribosomal S6 kinase beta
• p70 S6 Kinase beta
• p70 S6KB
• p70 S6K-beta
• p70(S6K)-beta
• p70-beta
• P70-beta-1
• P70-beta-2
• p70-S6K 2
• P70S6K2
• p70S6Kb
• ribosomal protein S6 kinase beta-2
• ribosomal protein S6 kinase, 70kD, polypeptide 2,70 kDa ribosomal protein S6 kinase 2
• ribosomal protein S6 kinase, 70kDa, polypeptide 2
• RPS6KB2
• S6K2
• S6K-beta
• S6K-beta2
• S6K-beta-2
• serine/threonine-protein kinase 14 beta
• Serine/threonine-protein kinase 14B
• SRK
• STK14B
• STK14BS6 kinase-related kinase

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KB2