p67phox/NOXA2 Antibody (JG86-31)

Western Blot: p67phox/NOXA2 Antibody (JG86-31) [NBP2-75605] - Analysis of NOXA2 on rat spleen tissue lysate using anti-NOXA2 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody (JG86-31) [NBP2-75605] - Analysis of paraffin-embedded rat spleen tissue using anti-NOXA2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody (JG86-31) [NBP2-75605] - Analysis of paraffin-embedded human spleen tissue using anti-NOXA2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody (JG86-31) [NBP2-75605] - Analysis of paraffin-embedded mouse spleen tissue using anti-NOXA2 antibody. Counter stained with hematoxylin.

Product Discontinued

p67phox/NOXA2 Antibody (JG86-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human p67phox/NOXA2 aa 180-320 / 526. (SwissProt: P19878 Human; SwissProt: O70145 Mouse; SwissProt: A7E3N2 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p67phox/NOXA2 Antibody (JG86-31)

• autosomal 2
• NADPH oxidase activator 2
• NCF2
• NCF-2
• neutrophil cytosol factor 2
• neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal2)
• neutrophil cytosolic factor 2
• NOXA2
• NOXA2FLJ93058
• p67phox
• p67-phox

Background

NOXA2 encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in two transcript variants that encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCF2