P2Y2 Overexpression Lysate (Native)

Images

 
Western Blot: P2Y2 Overexpression Lysate (Adult Normal) [NBL1-14044] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P2Y2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

P2Y2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223931 and based on accession number NM_176072. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens purinergic receptor P2Y, G-protein coupled, 2 (P2RY2), transcript variant 1, mRNA.
Gene
P2RY2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
42.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for P2Y2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for P2Y2 Overexpression Lysate (Native)

  • ATP receptor
  • HP2U
  • MGC20088
  • MGC40010
  • P2RU1
  • P2RY2
  • P2U nucleotide receptor
  • P2U purinoceptor 1
  • P2U
  • P2U1
  • P2UP2UR
  • P2Y purinoceptor 2
  • P2Y2
  • P2Y2P2Y2R
  • P2Y2R
  • purinergic receptor P2Y, G-protein coupled, 2
  • purinergic receptor P2Y2
  • Purinergic receptor
  • purinoceptor P2Y2

Background

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to both adenosine and uridine nucleotides. It may participate in control of the cell cycle of endometrial carcinoma cells. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript. Transcript variants 1, 2 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for P2Y2 Lysate (NBL1-14044) (0)

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Blogs on P2Y2.

P2Y2 (P2Y purinoceptor 2, ATP receptor)
The protein P2Y2 is a G-protein coupled metabotropic receptor that belongs to a larger family consisting of several receptor subtypes that each has a different pharmacological selectivity for various adenosine and uridine nucleotides. (This selecti...  Read full blog post.

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Bioinformatics

Gene Symbol P2RY2
Entrez