P2X4 Antibody

Western Blot: P2X4 Antibody [NBP1-00141] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Flow Cytometry: P2X4 Antibody [NBP1-00141] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

P2X4 Antibody Summary

• Description: Novus Biologicals Goat P2X4 Antibody (NBP1-00141) is a polyclonal antibody validated for use in WB, ELISA and Flow. Anti-P2X4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-YREKKYKYVEDYEQ corresponding to C-Terminus according to NP_002551.2.
• Epitope: C Terminus
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: P2RX4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Control Peptide:

  P2X4 Protein (NBP2-59721)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for P2X4 Antibody

• ATP receptor
• ATP-gated cation channel protein
• P2RX4
• P2X purinoceptor 4
• P2X4
• P2X4P2X receptor, subunit 4
• P2X4R
• purinergic receptor P2X, ligand-gated ion channel, 4
• Purinergic receptor
• purinoceptor P2X4

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2X4 Antibody (NBP1-00141)(2)

Publications using NBP1-00141

• Brown DA, Bruce JI, Straub SV et al. cAMP potentiates ATP-evoked calcium signaling in human parotid acinar cells. J Biol Chem 2004-09-01 [PMID: 15262999]
• Yeung D, Kharidia R, Brown SC, Gorecki DC. Enhanced expression of the P2X4 receptor in Duchenne muscular dystrophy correlates with macrophage invasion. Neurobiol Dis 2004-03-01 [PMID: 15006691]

Bioinformatics

• Gene Symbol: P2RX4
• Entrez:
  • 5025(Human)
  • 18438(Mouse)
  • 29659(Rat)
• Uniprot:
  • NP_002551.2(Human)