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![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining in human placenta and skeletal muscle tissues . Corresponding P2RX4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP1-92239-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining in human placenta and skeletal muscle tissues . Corresponding P2RX4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: P2X4 Antibody [NBP1-92239] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/P2X4-Antibody-Western-Blot-NBP1-92239-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: P2X4 Antibody [NBP1-92239] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92239-img0008.jpg)
![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human cerebral cortex shows positivity in neurons.](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP1-92239-img0010.jpg)
![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP1-92239-img0011.jpg)
![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP1-92239-img0012.jpg)
![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human small intestine shows positivity in luminal membrane in glandular cells.](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP1-92239-img0013.jpg)
![Western Blot: P2X4 Antibody [NBP2-27567] - Staining of A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/P2X4-Antibody-Western-Blot-NBP2-27567-img0004.jpg "Western Blot: P2X4 Antibody [NBP2-27567] - Staining of A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: P2X4 Antibody [NBP2-27567] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27567-img0003.jpg)
![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP2-27567] - Staining of paraffin embedded Human Uterus. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP2-27567-img0005.jpg)
![Western Blot: P2X4 Antibody [H00005025-B01P] - Analysis of P2RX4 expression in transfected 293T cell line by P2RX4 polyclonal antibody. Lane 1: P2RX4 transfected lysate(42.68 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/P2X4-Antibody-Western-Blot-H00005025-B01P-img0005.jpg "Western Blot: P2X4 Antibody [H00005025-B01P] - Analysis of P2RX4 expression in transfected 293T cell line by P2RX4 polyclonal antibody. Lane 1: P2RX4 transfected lysate(42.68 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: P2X4 Antibody [H00005025-B01P] - Analysis of negative control 293 cells (Black) and P2RX4 expressing 293 cells (Green) using P2RX4 purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/P2X4-Antibody-Flow-Cytometry-H00005025-B01P-img0004.jpg)
![Western Blot: P2X4 Antibody [H00005025-B01P] - analysis of P2RX4 expression in transfected 293T cell line by P2RX4 MaxPab polyclonal antibody. Lane 1: P2RX4 transfected lysate (42.68 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/P2X4-Antibody-Western-Blot-H00005025-B01P-img0001.jpg)
![Western Blot: P2X4 Antibody [NBP1-00141] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/P2X4-Antibody-Western-Blot-NBP1-00141-img0001.jpg "Western Blot: P2X4 Antibody [NBP1-00141] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: P2X4 Antibody [NBP1-00141] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/P2X4-Antibody-Flow-Cytometry-NBP1-00141-img0002.jpg)
![Western Blot: P2X4 Antibody [NBP3-38308] - Western blot analysis of various lysates using P2X4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38308_rabbit-p2x4-pab-1012202411421051.jpg "Western Blot: P2X4 Antibody [NBP3-38308] - Western blot analysis of various lysates using P2X4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: P2X4 Antibody [NBP3-38308] - Immunofluorescence analysis of MCF7 cells using P2X4 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38308_rabbit-p2x4-pab-101220241135308.jpg)
![Western Blot: P2RX4 Overexpression Lysate (Adult Normal) [NBP2-08102] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P2RX4.](http://images.novusbio.com/fullsize/P2RX4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08102-img0001.jpg "Western Blot: P2RX4 Overexpression Lysate (Adult Normal) [NBP2-08102] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P2RX4.")
![Western Blot: P2X4 Antibody [H00005025-D01P] - Analysis of P2RX4 expression in transfected 293T cell line by P2RX4 polyclonal antibody.Lane 1: P2RX4 transfected lysate(43.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/P2X4-Antibody-Western-Blot-H00005025-D01P-img0002.jpg "Western Blot: P2X4 Antibody [H00005025-D01P] - Analysis of P2RX4 expression in transfected 293T cell line by P2RX4 polyclonal antibody.Lane 1: P2RX4 transfected lysate(43.40 KDa).Lane 2: Non-transfected lysate.")