P2X3/P2RX3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: P2X3/P2RX3 Antibody [NB100-1658] - Staining in Dorsal Horn.
Immunohistochemistry-Paraffin: P2X3/P2RX3 Antibody [NB100-1658] - Non-petidergic fibers expressing P2X3 receptor for ATP are present in lamina II of the contralateral dorsal horn (arrows). The P2X3-positive fibers are ...read more
Immunocytochemistry/ Immunofluorescence: P2X3/P2RX3 Antibody [NB100-1658] - Staining in rat DRG.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Guinea Pig
Conjugate
Unconjugated

Order Details

P2X3/P2RX3 Antibody Summary

Immunogen
Synthetic peptide: VEKQSTDSGAYSIGH, corresponding to amino acids 383-397 of Rat P2X3.
Localization
Plasma membrane
Specificity
P2X3
Isotype
IgG
Clonality
Polyclonal
Host
Guinea Pig
Gene
P2RX3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Control
P2X3/P2RX3 Lysate (NBL1-14030)
Reviewed Applications
Read 1 Review rated 4
using
NB100-1658 in the following applications:

Publications
Read Publications using
NB100-1658 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID:24868547)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for P2X3/P2RX3 Antibody

  • ATP receptor
  • MGC129956
  • P2RX3
  • P2X purinoceptor 3
  • P2X receptor, subunit 3
  • P2X3
  • P2X3purinergic receptor P2X3
  • purinergic receptor P2X, ligand-gated ion channel, 3
  • Purinergic receptor
  • purinoceptor P2X3

Background

P2X3 is a subunit in the family of ion channels activated by extracellular ATP. It was cloned from rat dorsal root ganglia. P2X3 has two putative transmembrane domains with intracellular N- and C-termini. It can form homomeric channels and heteromeric channels with other members of the family. P2X3 is expressed in nociceptive sensory neurons

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P2X3/P2RX3 Antibody (NB100-1658)(3)

Review for P2X3/P2RX3 Antibody (NB100-1658) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-1658:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen P2X3/P2RX3 NB100-1658
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reviewed by:
Martin Helley
IHC-Fr Rat 08/05/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat trigeminal ganglion.
SpeciesRat
CommentsGood antibody, good results, minimal optimization.

Blocking

Blocking Details1 hour at room temperature with 10% normal donkey serum.

Primary Anitbody

Dilution RatioGuinea Pig anti-P2X3 (1:1000) incubated for 48hr at 4°C.

Secondary Antibody

Secondary Descriptiondonkey anti-guinea-pig conjugated to AF594
Secondary Manufacturer Cat#n/a
Secondary Concentration10µg/ml

Details

Detection NotesImaging performed with Nikon 80i epifluorescence microscope
Fixation DetailsPost-fix 4 hours in 4% paraformaldehyde. Permeabilized in 0.2% triton.
Wash Description3x 10 min washes in PBS between each step.

Comments

CommentsGood antibody, good results, minimal optimization.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for P2X3/P2RX3 Antibody (NB100-1658) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for P2X3/P2RX3 Antibody (NB100-1658)

Discover related pathways, diseases and genes to P2X3/P2RX3 Antibody (NB100-1658). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for P2X3/P2RX3 Antibody (NB100-1658)

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Research Areas for P2X3/P2RX3 Antibody (NB100-1658)

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Recent Reviews

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Martin Helley
08/05/2013
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol P2RX3
Entrez