p27/Kip1 Antibody (RM302)

Western Blot: p27/Kip1 Antibody (RM302) [NBP2-61571] - Western Blot of MCF-7 cells lysate using NBP2-61571.

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (RM302) [NBP2-61571] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using NBP2-61571.

Product Discontinued

p27/Kip1 Antibody (RM302) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A peptide corresponding to the C-terminus of Human p27/Kip1
• Specificity: This antibody reacts to human p27/Kip1. It may also react to mouse p27Kip1, as predicted by immunogen homology.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDKN1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS with 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for p27/Kip1 Antibody (RM302)

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN1B