p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
DCS-72.F6 + KIP1/769
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [DyLight 650] Summary

Immunogen
Mouse recombinant p27 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769)
Localization
Nuclear
Specificity
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [DyLight 650]

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47771AF350
Alexa Fluor 405 NBP2-47771AF405
Alexa Fluor 405 NBP2-44497AF405
Alexa Fluor 488 NBP2-47771AF488
Alexa Fluor 488 NBP2-44497AF488
Alexa Fluor 532 NBP2-47771AF532
Alexa Fluor 594 NBP2-47771AF594
Alexa Fluor 647 NBP2-47771AF647
Alexa Fluor 647 NBP2-44497AF647
Alexa Fluor 700 NBP2-47771AF700
Alexa Fluor 700 NBP2-44497AF700
Alexa Fluor 750 NBP2-47771AF750
Allophycocyanin NBP2-47771APC
Allophycocyanin NBP2-44497APC
Allophycocyanin/Cy7 NBP2-47771APCCY7
Biotin NBP2-47771B
Biotin NBP2-44497B
DyLight 350 NBP2-44497UV
DyLight 350 NBP2-47771UV
DyLight 405 NBP2-47771V
DyLight 405 NBP2-44497V
DyLight 488 NBP2-44497G
DyLight 488 NBP2-47771G
DyLight 550 NBP2-44497R
DyLight 550 NBP2-47771R
DyLight 594 NBP2-47771DL594
DyLight 650 NBP2-47771C
DyLight 650 NBP2-44497C
DyLight 680 NBP2-47771FR
DyLight 680 NBP2-44497FR
DyLight 755 NBP2-47771IR
DyLight 755 NBP2-44497IR
FITC NBP2-47771F
FITC NBP2-44497F
HRP NBP2-47771H
HRP NBP2-44497H
Janelia Fluor 549 NBP2-47771JF549
Janelia Fluor 646 NBP2-47771JF646
PE NBP2-47771PE
PE NBP2-44497PE
PE/Atto594 NBP2-47771PEATT594
PE/Cy5.5 NBP2-47771PECY55
PE/Cy7 NBP2-47771PECY7
PerCP NBP2-44497PCP
PerCP NBP2-47771PCP

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Bioinformatics Tool for p27/Kip1 Antibody (NBP2-44497C)

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Bioinformatics

Gene Symbol CDKN1B
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