p19 INK4d Antibody (2E10)

Western Blot: p19 INK4d Antibody (2E10) [H00001032-M08] - Analysis of CDKN2D expression in transfected 293T cell line by CDKN2D monoclonal antibody (M08), clone 2E10. Lane 1: CDKN2D transfected lysatE (17.7 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation: p19 INK4d Antibody (2E10) [H00001032-M08] - Analysis of CDKN2D transfected lysate using anti-CDKN2D monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CDKN2D rabbit polyclonal antibody.

Sandwich ELISA: p19 INK4d Antibody (2E10) [H00001032-M08] - Detection limit for recombinant GST tagged CDKN2D is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP, S-ELISA
• Clone: 2E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

p19 INK4d Antibody (2E10) Summary

• Immunogen: CDKN2D (AAH01822, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLLEEVRAGDRLSGAAARGDVQEVRRLLHRELVHPDALNRFGKTALQVMMFGSTAIALELLKQGVSPNVQDTSGTSPVHDAARTGFLDTLKVLVEHGADVNVPDGTGALPIHLAVQEGHTAVVSFLAAESDLHRRDARGLTPLELALQRGAQDLVDILQGHMVAPL
• Specificity: CDKN2D - cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) (2E10)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDKN2D
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p19 INK4d Antibody (2E10)

• CDK inhibitor p19INK4d
• cell cycle inhibitor, Nur77 associating protein
• cyclin-dependent kinase 4 inhibitor D p19
• cyclin-dependent kinase 4 inhibitor D
• cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
• inhibitor of cyclin-dependent kinase 4d
• INK4D
• p19
• p19-INK4D

Background

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN2D
• Entrez:
  • 1032(Human)
• Uniprot:
  • AAH01822(Human)