p15INK4b/CDKN2B Antibody

Product Discontinued

p15INK4b/CDKN2B Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 100-138 of Human p15 INK4b.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKN2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p15INK4b/CDKN2B Antibody

• CDK4I
• CDKN2B
• cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
• cyclin-dependent kinases 4 and 6 binding protein
• INK4BCDK4B inhibitor
• MTS2
• MTS-2
• MTS2CDK inhibitory protein
• Multiple tumor suppressor 2
• p14_CDK inhibitor
• p14_INK4B
• p14-INK4b
• p15 CDK inhibitor
• p15_INK4B
• P15cyclin-dependent kinase 4 inhibitor B
• p15INK4b
• p15-INK4b
• TP15

Background

The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFbeta-treated human keratinocytes, suggesting that p15 may act as an effector of TGFbeta-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFbeta-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p15INK4b/CDKN2B Antibody (NB100-91906)(2)

Publications using NB100-91906

• Huda N, Khambu B, Liu G et al. Senescence Connects Autophagy Deficiency to Inflammation and Tumor Progression in the Liver Cellular and molecular gastroenterology and hepatology 2022-04-08 [PMID: 35398596] (WB, IF/IHC, Mouse)
• Siddiqi S, Terry M, Matushansky I. Hiwi Mediated Tumorigenesis Is Associated with DNA Hypermethylatio PLoS ONE 2012-01-01 [PMID: 22438986] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: CDKN2B
• Entrez:
  • 1030(Human)
  • 12579(Mouse)
  • 25164(Rat)
• Uniprot:
  • P42772()