P-Rex1 Antibody (869704) [Unconjugated]

Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Rex1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for P-Rex1 at approximately 175 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 869704
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

P-Rex1 Antibody (869704) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human P-Rex1
  Lys1313-Arg1434
  Accession # Q8TCU6
• Specificity: Detects human P-Rex1 in ELISAs and Western Blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PREX1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Rex1 Antibody (869704) [Unconjugated]

• KIAA1415
• phosphatidylinositol 34,5-trisphosphate-dependent Rac exchanger 1 protein
• phosphatidylinositol-34,5-trisphosphate-dependent Rac exchange factor 1
• PRex1
• P-Rex1
• ptdIns(34,5)-dependent Rac exchanger 1

Background

PREX1 (phosphoinositol (3,4,5)-dependent Rac exchanger 1), also called P-Rex1, is a 185 kDa G-protein-activated guanine nucleotide exchange factor. It is mainly cytosolic, but also plasma membrane-associated, regulating cell motility and actin dynamics in neutrophils, migrating neurons, adipocytes and platelets. It is upregulated in cancers such as melanoma, prostate and breast. The 1659 amino acid (aa) human P-Rex1 contains DH, PH and tandem DEP GPCR signaling domains, and a PDZ scaffolding domain between aa 49-703. A 956 aa isoform with a 5 aa substitution for aa 1-703 is reported, and a 1561 aa isoform with a 91 aa substitution for aa 1471-1659 is predicted. The human P-Rex1 immunogen (aa 1313-1434) shares 94% and 95% aa sequence identity with mouse and rat P-Rex1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PREX1
• Uniprot:
  • Q8TCU6()