P-Rex1 Antibody (869704) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 869704
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

P-Rex1 Antibody (869704) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human P-Rex1
  Lys1313-Arg1434
  Accession # Q8TCU6
• Specificity: Detects human P-Rex1 in ELISAs and Western Blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Rex1 Antibody (869704) [Alexa Fluor® 532]

• KIAA1415
• phosphatidylinositol 34,5-trisphosphate-dependent Rac exchanger 1 protein
• phosphatidylinositol-34,5-trisphosphate-dependent Rac exchange factor 1
• PRex1
• P-Rex1
• ptdIns(34,5)-dependent Rac exchanger 1

Background

PREX1 (phosphoinositol (3,4,5)-dependent Rac exchanger 1), also called P-Rex1, is a 185 kDa G-protein-activated guanine nucleotide exchange factor. It is mainly cytosolic, but also plasma membrane-associated, regulating cell motility and actin dynamics in neutrophils, migrating neurons, adipocytes and platelets. It is upregulated in cancers such as melanoma, prostate and breast. The 1659 amino acid (aa) human P-Rex1 contains DH, PH and tandem DEP GPCR signaling domains, and a PDZ scaffolding domain between aa 49-703. A 956 aa isoform with a 5 aa substitution for aa 1-703 is reported, and a 1561 aa isoform with a 91 aa substitution for aa 1471-1659 is predicted. The human P-Rex1 immunogen (aa 1313-1434) shares 94% and 95% aa sequence identity with mouse and rat P-Rex1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.