OXTR Antibody

Images

 
Western Blot: OXTR Antibody [NBP1-28487] - (0.5ug/ml) staining of Rat Uterus (U) and Rat Skeletal Muscle (M) lysates (35ug protein in RIPA buffer). Data obtained from Maree Gould, University of Otago, New Zealand. ...read more
Immunocytochemistry/ Immunofluorescence: OXTR Antibody [NBP1-28487] - Staining of Human Prostate stromal cells. Overnight incubation at 4C and detected with Alexafluor secondary conjugated antibody.
Immunohistochemistry-Paraffin: OXTR Antibody [NBP1-28487] - (2ug/ml) staining of paraffin embedded Human Prostate Cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: OXTR Antibody [NBP1-28487] - Staining of lysates of PC3 cells treated in several ways (50,000 cells per lane). Reprobed for alpha-tubulin (50kDa) to serve as loading control (OXTR antibody incubation was ...read more
Immunohistochemistry-Paraffin: OXTR Antibody [NBP1-28487] - (5ug/ml) staining of PC3 cells. Detected by DAB. This data was obtained using a previous batch

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

OXTR Antibody Summary

Immunogen
Peptide with sequence C-RRLGETSASKKSN corresponding to C-Terminus according to NP_000907.2.
Epitope
C terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OXTR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3ug/ml
  • Immunocytochemistry/Immunofluorescence 5 - 10 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA detection limit 1:16000
Application Notes
IF: Human Prostate stromal cells with strong cytoplasm staining provided by Maree Gould, University of Otago, New Zealand. Data obtained from a previous batch (different goat).
ICC: PC3 cells with localized cytoplasm staining provided by Maree Gould, University of Otago, New Zealand. Data obtained from a previous batch (different goat).
WB: Approx. 75 kDa band observed in rat uterus lysates, but none in rat skeletal muscle lysates are reported in scietnific literature (PMID: 15459766).
IHC-P: Human prostate cancer shows staining of the stromal component of smooth muscle.
Control Peptide
OXTR Peptide (NBP1-28487PEP)
Publications
Read Publications using NBP1-28487.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for OXTR Antibody

  • OT-R
  • OXTR
  • oxytocin receptor

Background

The protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OXTR Antibody (NBP1-28487)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OXTR
Entrez
Uniprot