Otubain-1 Antibody (17E10) - BSA Free

Western Blot: Otubain-1 Antibody (17E10) [NBP2-59389] - Lane 1: A549 cell lysates, Lane 2: HeLa cell lysates, Lane 3: HepG2 cell lysates, Lane 4: 293T cell lysates

Immunocytochemistry/Immunofluorescence: Otubain-1 Antibody (17E10) [NBP2-59389] - Analysis of OTUB1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OTUB1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 17E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Otubain-1 Antibody (17E10) - BSA Free Summary

• Immunogen: Recombinant human Otubain-1 (1-271aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTUB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Otubain-1 Antibody (17E10) - BSA Free

• Deubiquitinating enzyme OTUB1
• EC 3.1.2
• EC 3.4.19.12
• FLJ20113
• FLJ40710
• hOTU1
• MGC111158
• OTB1
• OTB1MGC4584
• OTU domain, ubiquitin aldehyde binding 1
• OTU domain-containing ubiquitin aldehyde-binding protein 1
• OTU1
• OTU1OTU-domain Ubal-binding 1
• OTUB1
• Otubain1
• Otubain-1
• ubiquitin thioesterase OTUB1
• ubiquitin-specific protease otubain 1
• Ubiquitin-specific-processing protease OTUB1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUB1