OCRL Antibody (4A6) - Azide and BSA Free

Western Blot: OCRL Antibody (4A6) [H00004952-M02] - Analysis of OCRL expression in transfected 293T cell line by OCRL monoclonal antibody (M02), clone 4A6.Lane 1: OCRL transfected lysate(103.2 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: OCRL Antibody (4A6) [H00004952-M02] - Analysis of monoclonal antibody to OCRL on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.

Western Blot: OCRL Antibody (4A6) [H00004952-M02] - Analysis of OCRL over-expressed 293 cell line, cotransfected with OCRL Validated Chimera RNAi ( Cat # H00004952-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OCRL monoclonal antibody (M02), clone 4A6 (Cat # H00004952-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: OCRL Antibody (4A6) [H00004952-M02] - OCRL monoclonal antibody (M02), clone 4A6 Analysis of OCRL expression in Hela S3 NE.

Sandwich ELISA: OCRL Antibody (4A6) [H00004952-M02] - Detection limit for recombinant GST tagged OCRL is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, Mycoplasma
• Clone: 4A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

OCRL Antibody (4A6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: OCRL (NP_000267, 146 a.a. ~ 239 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGLLGFEDNFSSMNLDKKINSQNQPTGIHREPPPPPFSVNKMLPREKEASNKEQPKVTNTMRKLFVPNTQSGQREGLIKHILAKREKEYVNIQT
• Specificity: OCRL - oculocerebrorenal syndrome of Lowe
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OCRL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for OCRL Antibody (4A6) - Azide and BSA Free

• EC 3.1.3.36
• INPP5F
• LOCR
• Lowe oculocerebrorenal syndrome protein
• NPHL2
• OCRL1inositol polyphosphate 5-phosphatase OCRL-1
• oculocerebrorenal syndrome of Lowe
• phosphatidylinositol polyphosphate 5-phosphatase

Background

This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OCRL
• Entrez:
  • 4952(Human)
• OMIM:
  • 300009(Human)
• Uniprot:
  • NP_000267(Human)