O-GlcNAc Transferase p110 subunit Overexpression Lysate (Native)

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Western Blot: O-GlcNAc Transferase p110 subunit Overexpression Lysate (Adult Normal) [NBL1-13919] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for O-GlcNAc ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

O-GlcNAc Transferase p110 subunit Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206822 and based on accession number NM_181672.
Epitope
C-terminal DDK tag
Specificity
Homo sapiens O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1, mRNA.
Gene
OGT

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
116.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for O-GlcNAc Transferase p110 subunit

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for O-GlcNAc Transferase p110 subunit Overexpression Lysate (Native)

  • EC 2.4.1
  • EC 2.4.1.255
  • FLJ23071
  • HRNT1
  • MGC22921
  • O-GlcNAc transferase p110 subunit
  • O-GlcNAc transferase subunit p110
  • OGlcNAc Transferase
  • O-GlcNAc Transferase
  • O-GLCNAC
  • OGT
  • O-linked N-acetylglucosamine (GlcNAc) transferase(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
  • O-linked N-acetylglucosamine transferase 110 kDa subunit
  • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDasubunit
  • uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase

Background

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for O-GlcNAc Transferase p110 subunit Lysate (NBL1-13919) (0)

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Bioinformatics

Gene Symbol OGT
Entrez