NUDC Antibody

Western Blot: NUDC Antibody [NBP1-51971] - (0.5ug/ml) staining of HeLa (A), HepG2 (B), K562 (C) and KNRK (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-51971] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Rt, Mu, Bv, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NUDC Antibody Summary

• Description: Novus Biologicals Goat NUDC Antibody (NBP1-51971) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-NUDC Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PNLGNGADLPNYR corresponding to internal region according to NP_006591.1.
• Epitope: C-PNLGNGADLPNYR
• Predicted Species: Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NUDC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx 40kDa band observed in lysates of cell lines HeLa. HepG2 and K562, and approx. 38kDa in lysates of cell line KNRK (calculated MW of 38.2kDa according to Human NP_006591.1) and 38.4kDa according to Rat NP_058967.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NUDC Antibody

• HNUDC
• MNUDC
• NPD011
• nuclear distribution gene C (A.nidulans) homolog
• nuclear distribution gene C homolog (A. nidulans)
• Nuclear distribution protein C homolog
• nuclear migration protein nudC
• NudC

Background

NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates with dynein (see DYNC1H1; MIM 600112) (Aumais et al., 2003 [PubMed 12679384]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NUDC Antibody (NBP1-51971)(1)

Publication using NBP1-51971

• Tang YS, Zhang YP, Xu P. hNUDC promotes the cell proliferation and differentiation in a leukemic cell line via activation of the thrombopoietin receptor (Mpl). Leukemia 2008-05-22 [PMID: 18288130]

Bioinformatics

• Gene Symbol: NUDC
• Entrez:
  • 10726(Human)
  • 18221(Mouse)
  • 29648(Rat)
• Uniprot:
  • NP_006591.1(Human)