![Western Blot: NSUN4 Antibody [NBP2-19594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-19594-img0005.jpg "Western Blot: NSUN4 Antibody [NBP2-19594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit NSUN4 Antibody - BSA Free (NBP2-19594) is a polyclonal antibody validated for use in IHC and WB. Anti-NSUN4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NSUN4. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NSUN4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-19594 | Applications | Species |
|---|---|---|
| Yang L, Ren Z, Yan S et al. Nsun4 and Mettl3 mediated translational reprogramming of Sox9 promotes BMSC chondrogenic differentiation Communications Biology 2022-05-25 [PMID: 35614315] |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594] - Human colon carcinoma, using NSUN4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Immunohistochemistry-Paraffin-NBP2-19594-img0002.jpg "Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594] - Human colon carcinoma, using NSUN4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: NSUN4 Antibody [NBP2-19594] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-19594-img0001.jpg "Western Blot: NSUN4 Antibody [NBP2-19594] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: NSUN4 Antibody [NBP2-19594] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] diluted at 1:500.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-19594-img0003.jpg "Western Blot: NSUN4 Antibody [NBP2-19594] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] diluted at 1:500.")
![Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (NBP2-19594) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-19594_rabbit-polyclonal-nsun4-antibody-western-blot-26112025198361.jpg "Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (NBP2-19594) diluted at 1:500.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")