NSUN4 Antibodies
We offer NSUN4 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each NSUN4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NSUN4 Antibodies can be used in a variety of model species: Human. Use the list below to choose the NSUN4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NSUN4 polyclonal antibodies.
Alternate Names for NSUN4 Antibodies
anti-NSUN4 antibody, anti-EC 2.1.1 antibody, anti-EC 2.1.1.- antibody, anti-EC 2.1.1.29 antibody, anti-MGC22960 antibody, anti-NOL1/NOP2/Sun domain family 4 antibody, anti-NOL1/NOP2/Sun domain family member 4 antibody, anti-NOL1/NOP2/Sun domain family, member 4 antibody, anti-NOP2/Sun domain family, member 4 antibody, anti-putative methyltransferase NSUN4 antibody, anti-RP4-603I14.2 antibody
5 Results for "nsun4" in Primary Antibodies
![Western Blot: NSUN4 Antibody [NBP2-19594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-19594-img0005.jpg "Western Blot: NSUN4 Antibody [NBP2-19594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594] - Human colon carcinoma, using NSUN4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Immunohistochemistry-Paraffin-NBP2-19594-img0002.jpg)
![Western Blot: NSUN4 Antibody [NBP2-19594] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-19594-img0001.jpg)
![Western Blot: NSUN4 Antibody [NBP2-19594] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] diluted at 1:500.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-19594-img0003.jpg)
![Western Blot: NSUN4 Antibody [NBP2-19594] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] diluted at 1:500.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-19594-img0004.jpg)
![Western Blot: NSUN4 Antibody [NBP1-82733] - Analysis in control (vector only transfected HEK293T lysate) and NSUN4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP1-82733-img0007.jpg "Western Blot: NSUN4 Antibody [NBP1-82733] - Analysis in control (vector only transfected HEK293T lysate) and NSUN4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP1-82733] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Immunohistochemistry-Paraffin-NBP1-82733-img0006.jpg)
![Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP1-82733] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Immunohistochemistry-Paraffin-NBP1-82733-img0002.jpg)
![Western Blot: NSUN4 Antibody (4G3-R) [NBP3-32666] - Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (NBP3-32666) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32666) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32666_mouse-nsun4-mab-4g3-r-278202416285227.jpg "Western Blot: NSUN4 Antibody (4G3-R) [NBP3-32666] - Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (NBP3-32666) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32666) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NSUN4 Antibody (4G3-R) [NBP3-32666] - Immunocytochemistry analysis of MCF7 cells labeling NSUN4 with Mouse anti-NSUN4 antibody (NBP3-32666) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NSUN4 antibody (NBP3-32666) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32666_mouse-nsun4-mab-4g3-r-29202410334085.jpg)
![Western Blot: NSUN4 Antibody [NBP2-95156] - Analysis of extracts of various cell lines, using NSUN4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-NBP2-95156-img0001.jpg "Western Blot: NSUN4 Antibody [NBP2-95156] - Analysis of extracts of various cell lines, using NSUN4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: NSUN4 Antibody [H00387338-B01P-50ug] - NSUN4 MaxPab polyclonal antibody. Western Blot analysis of NSUN4 expression in human placenta.](http://images.novusbio.com/fullsize/NSUN4-Antibody-Western-Blot-H00387338-B01P-50ug-img0001.jpg "Western Blot: NSUN4 Antibody [H00387338-B01P-50ug] - NSUN4 MaxPab polyclonal antibody. Western Blot analysis of NSUN4 expression in human placenta.")