NSD3 Antibody


Western Blot: NSD3 Antibody [NBP1-04942] - Detection of NSD3 (isoform 1) in HeLa nuclear extracts using NBP1-04942.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF (-)
1 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

NSD3 Antibody Summary

Synthetic peptide made to an internal portion of the human NSD3 protein (within residues 300-400). [Swiss-Prot# Q9BZ95]
This has been observed to recognize a band that corresponds to isoform 1 of NSD3.
Predicted Species
Mouse (100%), Bovine (100%)
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This NSD3 antibody is useful for Western blot, where a band is seen at ~165 kDa corresponding to isoform 1 of NSD3.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
165 kDa

Reactivity Notes

Human. Predicted to react with mouse and bovine based on 100% sequence homology.

Alternate Names for NSD3 Antibody

  • EC
  • FLJ20353
  • histone-lysine N-methyltransferase NSD3
  • MGC126766
  • MGC142029
  • NSD3DKFZp667H044
  • Nuclear SET domain-containing protein 3
  • pp14328
  • Protein whistle
  • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
  • WHSC1-like protein 1
  • Wolf-Hirschhorn syndrome candidate 1-like 1
  • Wolf-Hirschhorn syndrome candidate 1-like protein 1


NSD family of SET domain-containing histone methyltransferases includes NSD1, NSD2 (also known as WHSC1, Wolf-Hirschhorn syndrome candidate 1 or MMSET, multiple myeloma SET), and NSD3 (also known as WHSC1L1, WHSC1 like 1). All the three NSD proteins have been directly linked to multiple human diseases and each NSD family member behaves as an oncogene. HSD3 is a istone methyltransferase that preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. Translocations resulting in NUP98 fusion to NSD1 and NSD3 leads to the development of acute myeloid leukemia (AML) and overexpression of NSD3 via gene amplification occurs in several breast cancer cell lines as well as in non small cell lung carcinomas (NSCLC).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: IHC-P
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB
Species: Hu
Applications: WB, ICC
Species: Mu
Applications: WB, Simple Western, IHC
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC-Fr
Species: Hu
Applications: WB, ELISA, S-ELISA

Publications for NSD3 Antibody (NBP1-04942) (0)

There are no publications for NSD3 Antibody (NBP1-04942).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NSD3 Antibody (NBP1-04942) (0)

There are no reviews for NSD3 Antibody (NBP1-04942). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for NSD3 Antibody (NBP1-04942): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NSD3 Antibody (NBP1-04942). (Showing 1 - 1 of 1 FAQ).

  1. I have a customer with several questions about the anti-WHSC1L1 antibody (catalog # NBP1-04942). They would like to know the following: Has this antibody been shown to detect the other three isoforms of this protein as shown on Swiss-Prot for Q9BZ95? They are expecting that it should detect the other isoforms because of the location of the immunogenic peptide. What was the exposure time for the Northern blot image shown on the datasheet? Has the antibody been tested in Western blot with any other cell lines or tissue extracts? May I just inquire did you try any additional cell lines or tissue extracts besides HeLa nuclear extracts? I agree with you that isoforms 1, 2 and 4 are very similar in size. Unfortunately, the customer specific asked mentioned isoform 3 which is missing amino acids 646-1437 and should therefore be much smaller. Might you have any testing data that shows detection of isoform 3? I admit I know nothing about which tissue types express which isoform other than what I see on Swiss-Prot.
    • The immunogen for NBP1-04942 is present in all isoforms of NSD3 so it should detect all of them. Most are very similar in size, so it may not be possible to distinguish them at such high molecular weights. For the Western blot, we do a variety of exposure times so I am not sure as to which one worked best for this particular image. It looks like we only tested this in HeLa, sorry! It also looks like a few of our test bleeds did detect isoform 3 at around 75 kDa but that wasn't the one we chose for production. As far as tissue specificity, I'm with you that I just have information from Swiss-Prot and the Human Protein Atlas which looks like this is pretty ubiquitous (Protein Atlas).

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP1-04942V
FITC Labeled NBP1-04942F

Additional NSD3 Antibody Products

Related Products by Gene

Bioinformatics Tool for NSD3 Antibody (NBP1-04942)

Discover related pathways, diseases and genes to NSD3 Antibody (NBP1-04942). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NSD3 Antibody (NBP1-04942)

Discover more about diseases related to NSD3 Antibody (NBP1-04942).

Pathways for NSD3 Antibody (NBP1-04942)

View related products by pathway.

PTMs for NSD3 Antibody (NBP1-04942)

Learn more about PTMs related to NSD3 Antibody (NBP1-04942).

Research Areas for NSD3 Antibody (NBP1-04942)

Find related products by research area.

Blogs on NSD3

There are no specific blogs for NSD3, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol WHSC1L1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-04942 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought