NSD3 Antibody

Western Blot: NSD3 Antibody [H00054904-B02P] - Analysis of WHSC1L1 expression in transfected 293T cell line by WHSC1L1 polyclonal antibody. Lane 1: WHSC1L1 transfected lysate(70.95 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NSD3 Antibody [H00054904-B02P] - Analysis of purified antibody to WHSC1L1 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: NSD3 Antibody [H00054904-B02P] - Analysis of WHSC1L1 expression in rat brain.

Product Discontinued

NSD3 Antibody Summary

• Immunogen: WHSC1L1 (NP_060248.2, 1 a.a. - 645 a.a.) full-length human protein. MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGSADRGVQGSVRFSDSSVSAAIEETVD
• Specificity: WHSC1L1 - Wolf-Hirschhorn syndrome candidate 1-like 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: WHSC1L1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NSD3 Antibody

• EC 2.1.1.43
• FLJ20353
• histone-lysine N-methyltransferase NSD3
• MGC126766
• MGC142029
• NSD3DKFZp667H044
• Nuclear SET domain-containing protein 3
• pp14328
• Protein whistle
• WHSC1-like 1 isoform 9 with methyltransferase activity to lysine
• WHSC1-like protein 1
• Wolf-Hirschhorn syndrome candidate 1-like 1
• Wolf-Hirschhorn syndrome candidate 1-like protein 1

Background

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NSD3 Antibody (H00054904-B02P)(1)

Publication using H00054904-B02P

• Saloura V, Vougiouklakis T, Zewde M et al. WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer Sci Rep 2017-01-19 [PMID: 28102297] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: WHSC1L1
• Entrez:
  • 54904(Human)
• Uniprot:
  • NP_060248.2(Human)