NRXN1 Antibody

Western Blot: NRXN1 Antibody [NBP1-00219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with (1/3000) in lane C. Mock-transfected HEK293 probed with NBP1-00219 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Flow Cytometry: NRXN1 Antibody [NBP1-00219] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NRXN1 Antibody Summary

• Description: Novus Biologicals Goat NRXN1 Antibody (NBP1-00219) is a polyclonal antibody validated for use in WB, ELISA and Flow. Anti-NRXN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KEKQPSSAKSSNKN corresponding to C-Terminus according to NP_620072.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_004792.1 and NP_620072.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRXN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5 - 1 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing full-length human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60 kDa band were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.
• Control:

  NRXN1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NRXN1 Antibody

• DKFZp313P2036
• FLJ35941
• KIAA0578Neurexin I-alpha
• neurexin 1
• neurexin I
• Neurexin I-beta
• neurexin-1-alpha
• neurexin-1-beta
• PTHSL2

Background

Neurexins function in the vertebrate nervous system as cell adhesion molecules and receptors. Two neurexin genes are among the largest known in human (NRXN1 and NRXN3). By using alternate promoters, splice sites and exons, predictions of hundreds or even thousands of distinct mRNAs have been made. Most transcripts use the upstream promoter and encode alpha-neurexin isoforms; fewer transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. Alpha-neurexins contain epidermal growth factor-like (EGF-like) sequences and laminin G domains, and they interact with neurexophilins. Beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. The RefSeq Project has decided to create only a few representative transcript variants of the multitude that are possible. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NRXN1 Antibody (NBP1-00219)(1)

Publication using NBP1-00219

• Feng J, Schroer R, Yan J et al. High frequency of neurexin 1beta signal peptide structural variants in patients with autism. Neurosci Lett 2006-11-27 [PMID: 17034946]

Bioinformatics

• Gene Symbol: NRXN1
• Entrez:
  • 9378(Human)
  • 18189(Mouse)
  • 60391(Rat)
• Uniprot:
  • Q9ULB1(Human)