NQO-1 Antibody (844142) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IP
• Clone: 844142
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

NQO-1 Antibody (844142) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human NQO-1
  Met1-Lys274
  Accession # P15559
• Specificity: Detects human NQO-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NQO-2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NQO-1 Antibody (844142) [Alexa Fluor® 594]

• Azoreductase
• DHQU
• DIA4
• DIA4azoreductase
• diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
• Diaphorase-4
• Dioxin-inducible 1
• DTD
• DTDEC 1.6.5.2
• DT-diaphorase
• Menadione Reductase
• NAD(P)H dehydrogenase [quinone] 1
• NAD(P)H dehydrogenase, quinone 1
• NAD(P)H:menadione oxidoreductase 1
• NAD(P)H:Quinone acceptor oxidoreductase type 1
• NAD(P)H:quinone oxidoreductase 1
• NAD(P)H:quinone oxireductase
• NMOR1
• NMOR1diaphorase-4
• NQO1
• NQO-1
• Phylloquinone reductase
• QR1
• QR1NMORI
• Quinone Reductase 1

Background

NQO-1 (NADPH Quinone acceptor Oxidoreductase 1; also DTD and Menadione reductase) is a 30-33 kDa cytoplasmic member of the NAD(P)H dehydrogenase family of enzymes. It is widely expressed, being found in adipocytes, endothelial cells and hepatocytes. NQO-1 serves as a cytoprotective molecule, reducing quinones (atmospheric benzene and esrtrogen compounds) and nitroaromatics without consuming sulfhydryl compounds. It also acts as a backup superoxide scavenger to SOD. NQO-1 reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome-mediated protein turnover. Human NQO-1 is 274 amino acids (aa) in length. It contains an FMN reductase domain (aa 5-212) and two utilized Lys acetylation sites. NQO-1 functions as a noncovalent homodimer. There are three potential at Met22, while two others show a deletion of aa 141-174 and 102-139, respectively. Full length human NQO-1 shares 86% aa sequence identity with mouse NQO-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.