NPM1 Antibody

ELISA: NPM1 Antibody [22000002] - Affinity Purified

Product Discontinued

NPM1 Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human NPM1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:2000
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Western Blot and Immunohistochemistry-Paraffin.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for NPM1 Antibody

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

The CD246 antigen, or ALK (Anaplastic Lymphoma Kinase; neural tyrosine kinase) is a 200 kDa transmembrane molecule expressed only in neural tissues (weakly) and by the rhabdomyosarcoma Rh30 cell line. The alk gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5)], the alk gene fuses with the npm gene. NPM, also known as nucleophosmin or B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre-ribosomal components between the nucleolus and the cytoplasm. The chimeric product resulting from t(2;5) translocation is a protein of 80 kDa with the N-terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SU-DHL-1 lymphoid cell lines strongly express NPM-ALK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPM1 Antibody (22000002)(2)

Publications using 22000002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: NPM1
• Uniprot:
  • NM_002520(Human)