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NPHP3 Antibody

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Western Blot: NPHP3 Antibody [NBP2-94783] - Analysis of extracts of various cell lines, using NPHP3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western Blot: NPHP3 Antibody [NBP2-94783] - Analysis of extracts of various cell lines, using NPHP3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NPHP3 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human NPHP3 (NP_694972.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPHP3
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NPHP3 Antibody

  • FLJ30691
  • FLJ36696
  • KIAA2000DKFZp667K242
  • MKS7DKFZp781K1312
  • nephrocystin-3
  • nephronophthisis 3 (adolescent)
  • NPH3MGC78666
  • RHPD

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Nephronophthisis Antibody [NBP2-94059] - Analysis of extracts of Mouse pancreas, using NPHP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Western Blot: Nephronophthisis Antibody [NBP2-94059] - Analysis of extracts of Mouse pancreas, using NPHP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: Nephronophthisis Antibody [NBP2-94059] - Rat kidney using NPHP1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Species: Hu, Mu, Rt
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NPHP4 Antibody
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Western Blot: NPHP4 Antibody [NBP2-93637] - Analysis of extracts of various cell lines, using NPHP4 .Exposure time: 30s.
Species: Hu, Mu, Rt
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Immunocytochemistry/Immunofluorescence: IQCB1 Antibody [NBP2-14126] - Staining of human cell line U-2 OS shows localization to nucleoplasm, microtubules, cytokinetic bridge & mitotic spindle.
Immunohistochemistry-Paraffin: IQCB1 Antibody [NBP2-14126] - Staining of human retina shows strong cytoplasmic positivity in cones.
Western Blot: IQCB1 Antibody [NBP2-14126] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
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Western Blot: CEP290 Antibody [NB100-86991] - Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody NB100-86991 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).
Western Blot: CEP290 Antibody [NB100-86991] - Representative examples of immunoblot images of purified olfactory cilia. Three distinct cilia preparations each from LRBA-KO and WT mice (age, 4 weeks) were blotted in parallel (10 ug protein each), along with an OE and a brain sample (50 ug protein each), all shown at the same exposure. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-017-08543-4) licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: RPGRIP1L Antibody [NBP1-84403] - Staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
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Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - NEK9 monoclonal antibody (M01), clone 1F6 Analysis of NEK9 expression in HeLa.
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Flow Cytometry: FALDH Antibody (2A7) [NBP2-01017] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FALDH antibody, and then analyzed by flow cytometry.
Western Blot: FALDH Antibody (2A7) [NBP2-01017] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FALDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FALDH.
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Immunohistochemistry-Paraffin: Neurexophilin 2 Antibody [NBP3-05854] - Staining of human NXPH2 in human brain with rabbit polyclonal antibody at 1:500 dilution.
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Flow Cytometry: A-RAF Antibody (JJ08-68) [NBP2-67843] - Analysis of Hela cells with A-RAF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Immunocytochemistry/Immunofluorescence: A-RAF Antibody (JJ08-68) [NBP2-67843] - Staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western Blot: Meckelin Antibody [NBP1-06590] - Immunodetection of Meckelin (120kDa) in whole cell extracts of HEK293 cells.
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Publications for NPHP3 Antibody (NBP2-94783) (0)

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Bioinformatics

Gene Symbol NPHP3