Nova1 Antibody (5D9)

Western Blot: Nova1 Antibody (5D9) [H00004857-M10] - Analysis of NOVA1 expression in PC-12 (Cat # L012V1).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 5D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nova1 Antibody (5D9) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NOVA1 (NP_002506, 408 a.a. ~ 507 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG
• Specificity: NOVA1 - neuro-oncological ventral antigen 1 (5D9)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOVA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Nova1 Antibody (5D9)

• neuro-oncological ventral antigen 1Nova-1
• Onconeural ventral antigen 1
• Paraneoplastic Ri antigen
• RNA-binding protein Nova-1
• Ventral neuron-specific protein 1

Background

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nova1 Antibody (H00004857-M10)(3)

Publications using H00004857-M10

• Wenchuan Q, Hongjuan F, Xinye L et al. Electroacupuncture at PC6 (Neiguan) Attenuates Angina Pectoris in Rats with Myocardial Ischemia-Reperfusion Injury Through Regulating the Alternative Splicing of the Major Inhibitory Neurotransmitter Receptor GABRG2. J Cardiovasc Transl Res. 2022-04-04 [PMID: 35377129]
• Lin JC, Lee YC, Liang YC et al. The impact of the RBM4-initiated splicing cascade on modulating the carcinogenic signature of colorectal cancer cells. Sci Rep 2017-03-09 [PMID: 28276498]
• Lin JC, Chi YL, Peng HY, Lu LH. RBM4–Nova1–SRSF6 splicing cascade modulates the development of brown adipocytes. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms 2016-08-12 [PMID: 27535496]

Bioinformatics

• Gene Symbol: NOVA1
• Entrez:
  • 4857(Human)
• Uniprot:
  • NP_002506(Human)