NMT2 Overexpression Lysate

Images

 
Western Blot: NMT2 Overexpression Lysate (Adult Normal) [NBL1-13695] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NMT2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NMT2 Overexpression Lysate Summary

Description
Transient overexpression lysate of N-myristoyltransferase 2 (NMT2) The lysate was created in HEK293T cells, using Plasmid ID RC202876 and based on accession number NM_004808. The protein contains a C-MYC/DDK Tag.
Gene
NMT2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NMT2 Overexpression Lysate

  • EC 2.3.1.97
  • glycylpeptide N-tetradecanoyltransferase 2
  • Myristoyl-CoA:protein N-myristoyltransferase 2
  • NMT 2
  • N-myristoyltransferase 2
  • Peptide N-myristoyltransferase 2
  • Type II N-myristoyltransferase

Background

N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-12963
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88204
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56708
Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
NBP1-06985
Species: Hu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
H00000302-M02
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IP, WB
NBP2-13674
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-82455
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF942
Species: Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NBP2-20214
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87518
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-128
Species: Hu, Mu, Rt
Applications: GS, ICC/IF, IHC, IHC-P
NBP2-61724
Species: Hu
Applications: ELISA, ICC/IF, WB
H00001958-M03
Species: Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-58200
Species: Hu, Ze
Applications: ICC/IF, IHC, IHC-P, WB
NB100-1919
Species: Ca, Fe, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF1356
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
NBL1-13695
Species: Hu
Applications: WB

Publications for NMT2 Lysate (NBL1-13695) (0)

There are no publications for NMT2 Lysate (NBL1-13695).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NMT2 Lysate (NBL1-13695) (0)

There are no reviews for NMT2 Lysate (NBL1-13695). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NMT2 Lysate (NBL1-13695) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NMT2 Products

Bioinformatics Tool for NMT2 Lysate (NBL1-13695)

Discover related pathways, diseases and genes to NMT2 Lysate (NBL1-13695). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NMT2 Lysate (NBL1-13695)

Discover more about diseases related to NMT2 Lysate (NBL1-13695).
 

Pathways for NMT2 Lysate (NBL1-13695)

View related products by pathway.

PTMs for NMT2 Lysate (NBL1-13695)

Learn more about PTMs related to NMT2 Lysate (NBL1-13695).

Blogs on NMT2

There are no specific blogs for NMT2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NMT2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NMT2