Ninjurin-2 Antibody [Unconjugated]

Ninjurin‑2 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Sheep Anti-Human/Mouse/Rat Ninjurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5056) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of human thymus, mouse thymus, rat thymus, NTera‑2 human testicular embryonic carcinoma cells and HT‑29 human colon adenocarcinoma cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Ninjurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5056) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ninjurin‑2 at approximately ~26kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Ninjurin-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Ninjurin-2
  Met1-Thr65
  Accession # Q9NZG7
• Specificity: Detects human, mouse, and rat Ninjurin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human Ninjurin-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NINJ2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ninjurin-2 Antibody [Unconjugated]

• FLJ56349
• Nerve injury-induced protein 2
• NINJ2
• ninjurin 2
• Ninjurin2
• Ninjurin-2

Background

Ninjurin-2 (nerve injury-induced protein 2) is a 20‑22 kDa member of the Ninjurin family of transmembrane (TM) proteins. It is expressed by multiple cell types, including Schwann cells, myenteric plexus and sensory neurons, and lymphocytes and participates in intercellular homophilic binding. Human Ninjurin-2 is 142 amino acids (aa) in length. It has an unusual membrane orientation. There is a 65 aa N-terminal extracellular domain (ECD) (aa 1‑65) that contains one phoshorylation site at Ser3, followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 128‑142). One potential alternate start site exists 46 aa upstream of the standard form start site. Over aa 1‑65, human Ninjurin-2 is 71% aa identical to mouse Ninjurin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ninjurin-2 Antibody (AF5056)(3)

Publications using AF5056

• Yuxia Sun, Xiang Chen, Cen Yue, Wenyi Yang, Shu Zhang, Zhimin Ou et al. Ninj2 regulates Schwann cells development by interfering laminin-integrin signaling Theranostics 2022-10-17 [PMID: 36438492]
• Yuxia Sun, Xiang Chen, Zhimin Ou, Yue Wang, Wenjing Chen, Tongjin Zhao et al. Dysmyelination by Oligodendrocyte‐Specific Ablation of Ninj2 Contributes to Depressive‐Like Behaviors Advanced Science 2022-01-01 [PMID: 34787377]
• Yuxia Sun, Xiang Chen, Zhimin Ou, Yue Wang, Wenjing Chen, Tongjin Zhao et al. Dysmyelination by Oligodendrocyte‐Specific Ablation of Ninj2 Contributes to Depressive‐Like Behaviors Advanced Science 2022-01-01 [PMID: 34787377] (Immunohistochemistry, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: NINJ2
• Uniprot:
  • Q9NZG7()