NIF3L1 Antibody (OTI5A7)

Western Blot: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NIF3L1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NIF3L1.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Human prostate tissue using anti-NIF3L1 mouse monoclonal antibody.

Western Blot: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIF3L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIF3L1.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NIF3L1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NIF3L1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NIF3L1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NIF3L1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Human endometrium tissue using anti-NIF3L1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Human Kidney tissue using anti-NIF3L1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NIF3L1 Antibody (OTI5A7) [NBP2-01317] - Staining of paraffin-embedded Human pancreas tissue using anti-NIF3L1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: OTI5A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.66 mg/ml

NIF3L1 Antibody (OTI5A7) Summary

• Immunogen: Full length human recombinant protein of human NIF3L1(NP_068596) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NIF3L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 38.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.66 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NIF3L1 Antibody (OTI5A7)

• ALS2CR1
• candidate 1
• MDS015
• NIF3 NGG1 interacting factor 3-like 1 (S. pombe)
• NIF3-like protein 1
• S.pombe homolog)-like 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NIF3L1
• Entrez:
  • 60491(Human)