Nicalin Antibody

Western Blot: Nicalin Antibody [NBP2-59116] - Whole cell lysate (50 ug) from HEK293T, LNCaP, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Nicalin Antibody [NBP2-59116] - Detection of mouse NCLN by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody NBP2-59116. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: Nicalin Antibody [NBP2-59116] - Detection of human NCLN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCLN antibody NBP2-59116 (lot NBP2-59116-2) used for IP at 20 ul/mg lysate. NCLN was also immunoprecipitated by a previous lot of this antibody (lot NBP2-59116-1) and another rabbit anti-NCLN antibody. For blotting immunoprecipitated NCLN, NBP2-59116 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Product Discontinued

Nicalin Antibody Summary

• Immunogen: Maps to a region between 375 and 425
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCLN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tris-buffered Saline containing 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Nicalin Antibody

• Ncl-1
• NCLN
• NET59
• nicalin homolog (zebrafish)
• nicalin homolog (zebrefish)
• Nicalin
• Nicastrin-like protein

Background

Nicastrin is an integral part of the Alzheimers associated gamma-secretase complex. Nicalin, a Nicastrin-like protein, is not associated with gamma-secretase but rather interacts with NOMO (Nodal modulator). Nodals are TGFbeta signaling factors that control various cell fate decisions during embryonic body planning stages in vertebrate development. The Nicalin/NOMO complex acts to regulate the Nodal signaling factors during gastrulation. This regulation most often affects the development of the axial mesoderm. Nodal signaling is an important factor for melanoma cell invasiveness and tumorigenicity and inhibition of this signal can promote melanoma cells reverting back toward a melanocyte phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCLN